Instrumentation

The Foods and Nutrition Laboratories are equipped with advanced analytical instruments for molecular, biochemical, and food analyses.

Proximate Composition Analysis

Nitrogen Analyzers: An Elementar rapid N exceed nitrogen analyzer is used for nitrogen and protein quantification based on the Dumas method. A Foss DT 208 Digestor and a Kjeltec 8200 distillation unit are available for nitrogen/protein analysis based on the Kjeldahl method.

Spectroscopy

Fourier-Transform Infra-Red (FTIR) Spectroscopy

Manufacturer:  Agilent

Model: 4300 handheld FTIR (spectral range: 4500-650 cm-1)

Chromatography

Fast Protein Liquid Chromatography (FPLC)

Manufacturer: GE Healthcare Life Sciences

Model: ÄKTA pure 25 L

Configuration: System pumps (up to 25 mL/min, 20 MPa), gradient mixer, injection valve, 280 nm UV detector, conductivity detector

Software: UNICORN 7

Accessory: Fraction collector, chromatography refrigerator

Media: CNBr-activated Sepharose 4B, Con A Sepharose 4B, DEAE Sepharose Fast Flow, Protein G Sepharose 4 Fast Flow, Sephacryl S-200 High Resolution, Sephacryl S-300 High Resolution

Application: Protein purification

High Performance Liquid Chromatography (HPLC)

Manufacturer: Waters

Model: Alliance e2695

Configuration: Waters 2998 Photodiode Array Detector, Waters 2475 Multiwavelength Fluorescence Detector

Software: Empower 3

Application: Chemical separations

Gas Chromatography/Mass Spectrometry-Olfactometry (GC/MS-O)

Manufacturer: Agilent

Model: 6890 Network GC, 5973 Turbo MSD

Configuration: Gerstel Olfactometer

Software: Chemstation G1701

Application: Odor detection and identification